AGFD 77 |
| Gastrodia elata, a member of the Orchidaceae family, has been used for an important herbal medicine for the treatments a range of symptoms, including neuralgia, headache, paralysis, and rheumatism in Asian countries. Fresh tuber of this plant is used as a food, and dried tuber powder is also used for an important ingredient in many dietary supplement products. A number of research reported that consumption of this plant have a various biological effects including anticonvulsant and neuromodulation. The phenolic compounds are regarded as biologically active constituents of this plant. The major phenolic chemical components are gastrodin, 4-hydroxyl benzyl alcohol, vanillin, and 4-hydroxybenzylaldehyde; however, systematic studies on antioxidant activity of these phenolic compounds have not well established. In this study, antioxidant properties of major phenolic components isolated from this plant will be discussed. Additionally, bioassay-guided isolation experiments were carried out to isolate potential antioxidant compounds. Dried rhizome of this plant was extracted with methanol and the resulting residues were subjected to partition with a different polarity of solvents (hexane, ethyl acetate, n-butanol, and water) for evaluation of their antioxidant activity using DPPH and ABTS assay methods. Among the different solvent fractions, the ethyl acetate fraction showed the strongest antioxidant activity. Further purification was carried out with column chromatographic methods. The structure of isolated phenoilc compounds was identified by 1H NMR, 13C NMR, 2D-NMR and MS techniques.
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General Posters
1:00 PM-3:00 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Poster
Division of Agricultural & Food Chemistry |