Sugar-immobilized gold nano-particles (SGNP): Novel bioprobe for the on-site analysis of the oligosaccharide-protein interactions

POLY 228

Yasuo Suda, ysuda@eng.kagoshima-u.ac.jp1, Yuko Kishimoto2, Tomoaki Nishimura1, Sakiko Yamashita2, Mina Hamamatsu1, Akihiro Saito1, Masaki Sato1, and Masahiro Wakao1. (1) Department of Nanostructure and Advanced Materials, Kagoshima University, 1-21-40, Kohrimoto, Kagoshima, Japan, (2) Sugar Chip R&D, Japan Science and Technology Agency, KIBC #421, 5-2-2, Minatojima-minami machi, Chuo-ku, Kobe, Japan
Our established immobilization technique was applied to gold nano-particle (GNP) for a novel on-site analytical bioprobe of the carbohydrate-protein interactions. A ligand conjugate containing α-D-glucopyranoside was reacted with GNP, followed by a dialysis to prepare α-D-glucopyranose immobilized GNP (Glcα4Glc-GNP). The color of Glcα4Glc-GNP in PBS solution was purple, showing plasmon absorption at 520 nm. When Glcα4Glc-GNP was incubated with Concanavalin A, the colloid solution became colorless quickly as nano-particles were aggregated. This change was dependent on the concentration of the protein and was detected visually. We have prepared so far over 30 kinds of sugar-immobilized GNP (SGNP). Applications of SGNP, such as kinetic analysis or quick isolation of the target protein from the mixture, will be also discussed.
 

7th International Biorelated Polymers Symposium
8:30 AM-12:40 PM, Monday, 11 September 2006 San Francisco Marriott -- Salon 14/15, Oral

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006