Dermatophytes are fungi that can invade the skin, and nails of a living host, and cause dermatophytosis like ringworm, tinea, etc. The objective of this study was to examine the antifungal activities of the extracts from liquid culture media of wood-rotting fungi against dermatophytes. Three dermatophytes, Trichophyton mentagrophytes, Microsporum gypseum, and Trichophyton rubrum, and three wood-rotting fungi, Cordyceps militalis, Laetiporus sulphureus var. Murill, and Gloeophyllum trabeum were used in this study. The liquid culture media without mycelia were extracted with chloroform, and then the chloroform fractions were concentrated by evaporation, and successively re-dissolved in distilled water and chloroform. The disc-diffusion assay was used to determine the antifungal activities. The results showed that the water-soluble extracts of G. trabeum had antifungal activities, with the highest against T. rubrum, while all chloroform-soluble extracts and the water-soluble extracts of C. militalis and L. sulphureus var. Murill were not effective against all dermatophytes.