PMSE 297 |
| Recently, the in vitro construction of cell-polymeric material composites for the treatment of skin or cartilage has been reported by adhesion and proliferation of cells on/in biodegradable matrices. However, 3-dimensional (3D) artificial tissues have not been achieve yet, because organs and tissues in the body are complicated organization of the appropriate components such as cells, extracellular matrix (ECM), and signal molecules. The cells and ECM have well organized and laminated 3D-structure in the micro- or nano-scales, for example, blood vessel, liver, and skin. To prepare the well organized 3D-artificail tissues, creation of novel 3D-cell assembly method is important. Here, we report our challenge for the 3D-cell assembly by layer-by-layer (LbL) technique. We prepared nano-meter sized fibronectin-gelatin layers (nano-ECM) onto surfaces of mouse L929 fibroblast cells or human umbilical artery smooth muscle cells (UASMC) monolayer in order to provide nano-meter sized scaffold on the first cell layer surface for adhesion of the second cell. The thickness of fibronectin-gelatin layer affected the adhesion of the second cell, and we successfully assembled 4 or 5 layers of L929 or UASMC. The 3D-cell assembly method would have a possibility to provide well organized 3D-artificial tissues. |
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Joint PMSE/POLY Poster Session
6:00 PM-8:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Division of Polymeric Materials: Science & Engineering |