In order to make a comprehensive evaluation of the quality, including the nutrition and toxicity, of chromium-enriched eggs, the contents of Cr(III) and Cr(VI) were determined by graphite furnace atomic absorption spectrometry (GF-AAS). Thenoyltrifluoracetonate (TTA) was used to form volatile complex with Cr(III) which could be separated from Cr(VI) as a vapor in the graphite furnace. The total content of Cr in the eggs was determined by GF-AAS without adding any TTA, whilst the content of Cr(VI) was determined by GF-AAS with TTA. The content of Cr(III) was then calculated as the difference between the total content of Cr and the content of Cr(VI). In this study, the effects of pH, concentration of TTA, sonication time and temperature on the formation of Cr(III)-(TTA)₃ volatile complex had been investigated. The effects of the atomization conditions on the separation of Cr(VI) and Cr(III) in graphite furnace were discussed. The contents of total Cr in these eggs were in the range of 0.096 to 0.15 mg/kg, the contents of Cr(VI) were below the limit of quantification of this method which was 0.003 mg/kg.Both of the contents of total Cr and Cr(VI) were below their maximum residue levels (MRL) available.