Background reduction for surface assays in optically dense samples using surface plasmon-coupled emission

ANYL 264

Evgenia G. Matveeva, ematveev@hsc.unt.edu1, Ignacy Gryczynski2, Julian Borrejdo3, Anne Barnett3, and Zygmunt Gryczynski, zgryczyn@hsc.unt.edu1. (1) Department of Molecular Biology and Immunology, University of North Texas, Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, (2) Department of Cell Biology and Genetics, University of North Texas, Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, (3) University of North Texas, Health Science Center
We present a new approach for performing fluorescence assays in optically dense samples on a metal-coated surface. This approach, which is based on Surface Plasmon-Coupled Emission (“SPCE”), provides increased sensitivity and substantial background reduction due to exclusive selection of the signal from the fluorophores located near a bio-affinity surface. We detected the SPCE fluorescence signal of the fluorophore-labeled antibodies bound to the surface-immobilized antigen in absence and presence of optically dense media, and compared the effect of such media on the value of the signal. Input of small (low molecular weight dyes) and large (red blood cells, dyed beads) species in the media is discussed. We believe that SPCE has the potential of becoming a powerful approach for performing surface-based assays directly in dense samples such as whole blood, with no need for washing steps.
 

Analytical Approaches, Spectroscopy
8:30 AM-12:00 PM, Wednesday, 13 September 2006 Moscone Center -- Room 124, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006