PHYS 333 |
| Genes coding for a functional mRNA that contains 24 repeats of the MS2 binding motif combined with a MS2 coat protein fusion to GFP allowed us to track single molecules of RNA in live cells. The analysis demonstrated that nuclear RNA mobility was not directed, but governed by rules of simple diffusion. Once in the cytoplasm, mRNAs can be directed to their destinations. beta-actin mRNA can be localized to sites of active F-actin polymerization in migrating fibroblasts or growing neurons. It is possible to observe this process directly in real time in order to deduce the mechanisms governing localization. The RNA binding protein, ZBP1 contributes to the asymmetric sorting of beta-actin by inhibiting its translation until it reaches its destination. Disruption of this process severely affects the extension of neurites in primary hippocampal cells. By use of the variety of technologies developed, we can envision following an RNA molecule from its birth through its travels and finally to its expression. |
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Frontiers in Single-Molecule Biophysical Chemistry and Imaging
1:20 PM-5:40 PM, Wednesday, 13 September 2006 Grand Hyatt San Francisco -- Plaza Ballroom West, Oral
Division of Physical Chemistry |