ANYL 195 |
| The effects of the nephrotoxin gentamicin on 25, 40, and 80 day old male, Sprague Dawley rats were investigated using NMR-based and LCMS-based metabonomics approaches. At each age, groups of 4 rats were administered saline, 50 mg/kg gentamicin, or 100 mg/kg gentamicin. Predose urine was collected along with urine at 8, 24, 48, and 72 hours after dosing for metabonomics analysis. Principle component analysis (PCA) of the binned NMR or MS spectra was performed separately in order to investigate patterns of changes as a function of time for each age group. The PC plots show clustering based on age, dose, and time of urine collection. Metabolic biomarkers of gentamicin-induced drug efficacy and drug toxicity were found in both the NMR and MS data. The use of heteroscopic correlation analysis between NMR and MS data was able to identify the biomarkers. Finally, the FDA's Critical Path Initiative and the voluntary submission of ‘omics'data to the FDA will be presented. |
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Development and Applications of Metabonomic/Metabolomic Methods of Analysis
8:30 AM-11:35 AM, Monday, 11 September 2006 Moscone Center -- Room 124, Oral
Division of Analytical Chemistry |