AGFD 65 |
| In order to model the milk fat globule membrane (MFGM), we have studied the formation of solid-supported lipid bilayers (SLB) derived from MFGM lipids deposited on solid substrates by the Langmuir-Blodgett (LB) technique and adsorption driven liposome rupture. The morphology of the bilayer films formed via each technique was explored with atomic force microscopy (AFM). The thermodynamics and phase behavior of lipid monolayers were also studied via surface pressure – area compression isotherms on an aqueous subphase. Liposomes were created by exposure of MFGM lipids to high-pressure homogenizer. The rupture of liposomes and bilayer formation was characterized by quartz crystal microbalance (QCM). The MFGM lipids, naturally heterogeneous in composition, showed different morphology by deposition technique – organizing into phase-separated domains and forming other surface structures. This reflected the response of the phospholipids to the preparation methods, namely the high-pressure environment used to form liposomes, and difference in the bilayer formation processes. |
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General Posters
1:00 PM-3:00 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Poster
Division of Agricultural & Food Chemistry |