Chemical and physical evaluation of the crosslinking of bovine hide collagen by genipin

AGFD 89

Keyi Ding1, Maryann M. Taylor2, and Eleanor M. Brown, ebrown@errc.ars.usda.gov2. (1) ERRC, ARS, USDA and SWUN, China, 600 East Mermaid Lane, Wyndmoor, PA 19038, (2) Fats, Oils and Animal Coproducts Research Unit, ERRC, ARS, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038
Genipin, a derivative of the iridoid glycoside geniposide in the fruit of Gardenia jasmindides Ellis, is a naturally occurring protein crosslinking agent, which is beginning to replace glutaraldehyde as a fixative for biological tissues. In this study the potential of genipin, alone or in combination with aluminum, for use as a tanning agent on bovine hide powder and pieces of intact hide was explored. Parameters including the dosage of genipin, reaction time, pH and temperature of the tanning process were varied. Lysine crosslinks were estimated by comparing the available amine groups before and after reaction with genipin. Increased thermal stability, a typical measure of tanning effectiveness, was observed. The melting, or shrinkage temperature of 63 °C for untreated hide powder was increased to 79 °C for hide powder treated with 5% genipin at pH ~ 7 and 35 °C for 24 h. Thermal stability approaching 100 °C, the standard for chrome tanned leather, was achieved when genipin was used in combination with aluminum.
 

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Division of Agricultural & Food Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006