Nanoparticle labels for signal amplification in electrochemical immunoassays

ANYL 298

Yuehe Lin, yuehe.lin@pnl.gov, Jun Wang, Guodong Liu, and Hong Wu, hong.wu@pnl.gov. Pacific Northwest National Laboratory, Richland, WA 99352
Sensitive electrochemical immunoassys based on nanoparticle labels has been developed for the detection of protein biomarker, biomarker of recombinant human tumor necrosis factor-a(TNF-a). The use of functional quantum dots, carbon nanotubes, and silica nanoparticles as labels for signal amplification will be discussed. This method is simple, selective, and reproducible for trace biomarker analysis. A remarkable LOD has been achieved through signal amplification by functionalized nanoparticle labels. The work demonstrates the feasibility of developing an inexpensive, sensitive, and portable device for multiplexed diagnoses of different protein biomarkers.