Understanding the structures and dynamics of gas-phase protein ions and applications for whole proteome analysis: The aging of Drosophila and mapping human plasma

ANYL 225

David E. Clemmer, clemmer@indiana.edu, Department of Chemistry, Indiana University, Bloomington, IN 47405
The mobility of an ion through a buffer gas, under the influence of a uniform field depends upon the ion's shape and charge, as well as details associated with the ion neutral collisions. This talk focuses on two aspects of mobility measurements: 1) the use as a structural probe of proteins, peptides and amino acid clusters; and 2) the inclusion of an additional mobility dimension for analysis of complex mixtures of proteins encountered in proteomics. Several examples will be presented, including: 1) changes in protein ion structure that appear to occur as ions are stored in paul trap; 2) the role of chirality in assembling amino acid clusters; and, 3) the application of these technologies to the human plasma proteome and the aging proteome of a model organism (Drosophila melanogaster, the fruit fly). Finally, directions for future development of these techniques will be discussed.