AGFD 87 |
| Dangyooja(Citrus grandis Osbek) is one of Korean citrus fruit. Dangyooja is similar to yuzu in appearance, although it is bigger than yuzu due to its thick layer of peel. Traditionally, Dnagyooja has been a good source of medicines for the relief of cough and ulcer in Korea. Diverse flavonoids, which can exert health promoting properties such as antioxidant, antiinflammatory, antiallergic, anticarcinogenic and antiulcer effect, have been found in various citrus fruits. The objective of this study was to determine flavonoids in Dangyooja. Flavonoids were extracted from dried Dnagyooja by ethanol, followed by evaporation to dryness before redissolved in methanol. A reversed-phase high performance liquid chromatography was used for separation and determination of flavonoids in different tissues of Dangyooja. Flavonoids were eluted from a C-18 reverse-phase column using tetrahydrofuran /water /acetic acid (21:72:2, v/v/v) as the isocratic mobile phase. By comparing UV-Vis spectra and retention times of unknowns with those of authentic standards, three flavonoids such as hesperidin, neohesperidin, and naringin were identified. The contents of flavonoids were 0.9 mg/g for hesperidin, 11.1 mg/g for neohesperidin, and 4.4 mg/g for naringin in the pulp of mature Dangyooja. On the other hand, the peel was found to contain 1.5 mg/g for hesperidin, 23.1 mg/g for neohesperidin, and 14.8 mg/g for naringin, respectively. In addition, the contents of hesperidin (12.2 mg/g), neohesperidin (41.4 mg/g), and naringin (31.2 mg/g) were higher in the premature fruit than those in the mature fruit which had 1.5 mg/g for hesperidin, 23.7 mg/g for neohesperidin, and 13.9 mg/g for naringin. |
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General Posters
1:00 PM-3:00 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Poster
Division of Agricultural & Food Chemistry |