DNA templated Ag nanocluster formation

PHYS 512

Caroline M. Ritchie, caroline.ritchie@furman.edu1, Spence T. Gibbs1, John R. Kiser1, Robert M. Dickson, dickson@chemistry.gatech.edu2, and Jeffrey T. Petty, jeff.petty@furman.edu1. (1) Department of Chemistry, Furman University, 3300 Poinsett Highway, Greenville, SC 29613, (2) School of Chemistry and Biochemistry, Georgia Institute of Technology, 770 State Street, Atlanta, GA 30332-0400
The affinity of silver cations for DNA bases has enabled the creation of short oligonucleotide-encapsulated silver nanoclusters without the formation of large nanoparticles. Previous studies suggest that base specific interactions are important for these nanoclusters, and this work describes studies with oligonucleotides of cytosine. The position and intensity of the fluorescence bands depend on the pH of the solution, suggesting that the nanoclusters are associated with the heterocyclic bases. The development of some fluorescence bands at the expense of others is consistent with the formation of nanoclusters with differing silver stoichiometries. From mass spectra, the nanoclusters are associated with the oligonucleotides and have 1-6 silver atoms, with the size increasing over time. Fluorescence correlation spectroscopy was used to measure the concentration and diffusion coefficient of the nanoclusters-oligonucleotide conjugates.

Poster Session
7:30 PM-10:00 PM, Wednesday, 13 September 2006 Moscone Center -- Hall D, Poster

8:00 PM-10:00 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Sci-Mix

Division of Physical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006