ANYL 6 |
| Monolithic porous polymers are in their teenage. Being introduced in the late 1980s, they developed from an academic curiosity into commercial products and found a wide variety of applications including chromatography. Composition of the polymerization mixture is an excellent and simple tool to control both surface chemistry desired for separations in typical chromatographic modes and porous properties required for flow through at reasonable back pressure. Alternatively, a generic monolith with optimized porous structure is prepared first and the chemistry grafted onto pore surface in the second step. Since the entire mobile phase must flow through the monolithic stationary phase, mass transport of the separated compounds to the interacting functionalities at the pore surface is achieved by convection. In contrast to diffusion, which is typically slow for large molecules, convection is rapid and enables to achieve very fast separations. The performance of monolithic columns will be demonstrated with extremely rapid separations of proteins and synthetic polymers. Monolithic technology also enables other extreme – miniaturization of the separation devices to micro- and even nanoformats. Examples of these applications will also be presented. |
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Extreme Chromatography and Separations
8:25 AM-12:00 PM, Sunday, 10 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |