Removal of alkylphenols by the combined use of mushroom or Aspergillus oryzae tyrosinase and chitosan beads

POLY 430

Kazunori Yamada, k5yamada@cit.nihon-u.ac.jp1, Ayumi Kashiwada1, Kiyomi Matsuda1, Mitsuo Hirata1, Yukihiro Nakamura, yukihiro@gekkeikan.co.jp2, Yoji Hata, y_hata@gekkeikan.co.jp2, and Katsuya Higashida, higashida@gekkeikan.co.jp2. (1) Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, 1-2-1 Izumi-cho, Narashino, Chiba, 275-8575, Japan, (2) Research Institute, Gekkeikan Sake Co. Ltd, 101 Shimotoba-koyanagi-cho, Fushimi-ku, Kyoto, 612-8361, Japan
We investigated removal of p-alkylphenols from aqueous solutions through the two-step approach, the quinone conversion of p-alkylphenols with tyrosinases from mushroom or Aspergillus oryzae and the subsequent adsorption of quinones generated on the chitosan beads at pH 7.0 and 45 degrees C for mushroom tyrosinase and at pH 6.0 and 30 degrees C for Aspergillus oryzae tyrosinase. For some p-alkylphenols, the % removal values increased by increasing either the tyrosinase concentration or the amount of added chitosan beads. The % removal values of 95-100 % were obtained. This technique is quite useful for removal of various p-alkylphenols from an aqueous solution. This technique is much more effective than other methods repeated previously and is an easy-to-operate procedure, since the chitosan beads are readily separable from the reaction solutions after removal of p-alkylphenols. This technique was applicable to remove 4-n-octylphenol and 4-n-nonylphenol as suspected endocrine disrupting chemicals.
 

7th International Biorelated Polymers Symposium
6:00 PM-8:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster

Division of Polymer Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006