Construction of 20-µL microflow NMR probe with laser-etching for high-throughput chemical analysis

ANYL 261

Ian D. Henry, ihenry@purdue.edu, Gregory H. J. Park, ghjpark@purdue.edu, and Daniel Raftery, raftery@purdue.edu. Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907-2084
The analysis of small biological samples by NMR is facilitated by matching the detection coil volume to the available sample. We constructed a 20 μL microflow NMR probe that uses a horizontal flow cell, a solenoid coil around this sample cell, and a pair of microcapillaries that deliver a series of samples through the flow cell. The sample cell was fabricated from a thick-walled glass capillary tube. Laser etching of the sample cell was accomplished by flowing concentrated hydrofluoric acid through this capillary tube, while it was heated locally by a carbon dioxide laser and cooled by cryogen-cooled air. This process allowed the fabrication of the sample cell with the desired size and shape.
 

Analytical Approaches, Spectroscopy
8:30 AM-12:00 PM, Wednesday, 13 September 2006 Moscone Center -- Room 124, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006