ANYL 324 |
| Near-infrared (NIR) spectroscopy offers a painless and convenient method for measuring glucose concentrations noninvasively, thereby enhancing frequent diabetes monitoring and delaying medical complications. The work presented here demonstrates the potential of noninvasive ultrafiltrate glucose measurements by analyzing samples collected from bovine blood. Blood samples were passed through a KrosFlo hollow fiber ultrafiltration module coupled with a Materflex peristaltic pump. Standard solutions were prepared with randomized concentrations of glucose, urea and triacetin in blood ultrafiltrate. NIR spectra were collected over the combination spectral region with Nicolet Nexus 670 Fourier transform spectrometer. Single beam and absorbance spectra were used to build multivariate calibration models for glucose and urea. Results demonstrate excellent calibration performance for measuring glucose and urea. Pure component selectivity analysis and correlations between pure component spectra and the corresponding PLS regression vectors indicate the calibration models built from single beam and absorbance spectra are based specifically on glucose spectral information. |
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Analytical Approaches: Sensors
1:30 PM-4:20 PM, Thursday, 14 September 2006 Moscone Center -- Room 123, Oral
Division of Analytical Chemistry |