Disposable POD depth filter evaluation

BIOT 300

Francisco Valles1, Megumi Noguchi2, Paul Bezy3, Adema Arroyo1, Brad Wolk1, and Marty Siwak2. (1) Process Development Engineering, Genentech, Inc, 1 DNA Way, South San Francisco, CA 94080, (2) Process Technology Group, Millipore Corporation, 900 Middlesex Turnpike, Billerica, MA 01821, (3) Process Development Operations, Genentech, Inc, 1 DNA Way, South San Francisco, CA 94080
Depth filtration is a separation technology commonly used in whole cell clarification. Lenticular depth filters are the standard configuration used in this operation. This configuration requires assembly, cleaning, and steaming operations. To eliminate these requirements, Pod filters have been developed by Millipore Corporation. This presentation summarizes the evaluation of Pod filters on whole cell clarification. The Pod system's whole-cell clarification pmax capacity was determined using different material sources. Data will be presented at different scales, including the bench scale systems 0.002 m2 and 0.03 m2, as well as the industrial scale system ranging from 0.1 m2 to 1.0 m2. Comparisons between the POD and lenticular system will be discussed.
 

Poster Session
5:30 PM-7:30 PM, Wednesday, 13 September 2006 Moscone Center -- Hall D, Poster

Division of Biochemical Technology

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006