A (narrow-bore) pipeline for membrane protein structural biology

BIOT 116

Michael C. Wiener, mwiener@virginia.edu, Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908-0736
Research in our laboratory is directed towards the structural biology of integral membrane proteins. We have pursued (and are pursuing) active methods-development efforts in multiple areas of membrane protein structural biology, e.g., expression, purification, characterization, crystallization and crystallography. Many aspects of these individual areas can be viewed as screening steps, and the assembly of these areas into a tractable manageable whole constitutes a process (or pipeline). Elements of this (narrow-bore) pipeline will be described. Current foci of the lab are bacterial TonB-dependent outer membrane transport, and human integral membrane proteins of biomedical & biotechnological interest.