Screening of DNA sequences with high affinity for proteins involved in signal transduction by capillary electrophoresis: Systematic evolution of ligands by exponential Enrichment (CE-SELEX)

ANYL 141

Sam F Y Li, chmlifys@nus.edu.sg, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore
In Systematic Evolution of Ligands by Exponential Enrichment (SELEX), DNA or RNA sequences with high affinity for targets (e.g. proteins) are selected from a random library of sequences based on their affinities to the target molecules. Conventional SELEX screening procedures are time consuming and laborious, requiring many iterations (typically more than 10), each involving several purification steps (e.g. filtering, panning, affinity chromatography, etc). By using capillary electrophoresis to perform SELEX, much faster selection (2 to 3 iterations) can be achieved due to its high resolving power and short analysis time. The CE-SELEX procedure was applied to the screening of DNA fragments to identify sequences with high affinity to proteins involved in signal transduction. The results show that rapid screening can be achieved with this alternative technique.
 

General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006