Development of a flow injection – capillary electrophoresis (FI-CE) system using fiber optic detection

CHED 233

Froseen T. Dahdouh1, Florence Tse1, Keith Clarke1, Grady Hanrahan, ghanrah@calstatela.edu2, and Frank A. Gomez, fgomez2@calstatela.edu2. (1) Department of Chemistry & Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032, (2) Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032
Noteworthy developments in the design of a flow injection- capillary electrophoresis (FI-CE) instrument and applications to biological and environmental aspects are illustrated. Capillary electrophoresis (CE) is an excellent separation technique for countless species including antibiotics, proteins and inorganic anions. Even though there are many advantages of CE, it suffers from discontinuous and biased sample introduction. However, this can be resolved by coupling CE with flow injection (FI), an efficient sample introduction technique. CE separates molecules based on differences in electrophoretic mobilities under the influence of an applied electric field. Using FI as a continuous pumping mechanism, sample and reagents are injected into the carrier stream that propels the sample zone through a detection system and on to waste. By combining CE with FI, sample introduction is reliable with higher precision and reproducibility. FI-CE also offers online pretreatment techniques such as filtration, sample dilution and preconcentration. Future work on the development of FI-CE in a microfluidic format is underway
 

Undergraduate Research Poster Session: Chemical Education
2:30 PM-4:30 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Poster

Division of Chemical Education

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006