Thermodynamics and pH for the biological buffers MES and TRICINE from 5 to 55°C

CHED 205

Chandra N. Roy, croy@drury.edu, Sean R. LeNoue, slenoue@drury.edu, Cole E. Denton, cdenton@drury.edu, Paul A. Bryant, pbryant@drury.edu, Daniel A. Kaufmann, dkaufmann@drury.edu, Lakshmi N. Roy, lroy@drury.edu, and Rabindra N. Roy, rroy@drury.edu. Walter H. Hoffman Department of Chemistry, Drury University, 900 N. Benton Ave., Springfield, MO 65802
The pK2 and pH values for zwitterionic amino acids MES and TRICINE have been determined at temperatures from 5 to 55°C by the electromotive-force method using the cell: Pt; H2(g, 1 atm) | MES (m1), NaMESate (m2), NaCl (m3) AgCl, Ag. In all, four buffer solutions have been studied: (i) 0.04 m MES + 0.04 m NaMESate; (ii) 0.08 m MES + 0.08 m NaMESate + 0.08 m NaCl; (iii) 0.04 m TRICINE + 0.04 m NaTRICINate; and (iv) 0.08 m TRICINE + 0.08 m NaTRICINate + 0.08 m NaCl. These buffer compounds have been recommended for standard biochemical buffers in clinical studies.
 

Undergraduate Research Poster Session: Biochemistry
2:30 PM-4:30 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Poster

Division of Chemical Education

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006