ANYL 150 |
| Microfluidic devices (MDs) are powerful tools for performing an array of applications. A major challenge associated with the desired scales of MDs is to simultaneously reduce the number of pipetting steps needed to load the devices while amortizing the sample volume over several steps. Capillary electrophoresis (CE) is a technique that has shown great promise when coupled to microfluidic devices. Although CE has gained widespread use because of its versatility there is still the need to prepare samples at variable concentrations which slows down the analysis. Herein, we describe our work on coupling affinity CE (ACE) to "lab-through-a-chip" devices using the binding of arylsulfonamides to carbonic anhydrase B (CAB, EC 4.2.1.1) and peptides to vancomycin. Using multilayer soft lithography (MSL), fluid and control channels are fabricated to allow for manipulation of material on the device without the need of time-consuming pipetting steps. Subsequent electrophoresis using CE affords separation of materials. |
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |