ANYL 142 |
| This research demonstrates the development and application of new methods using capillary isotachophoresis coupled with NMR detection (cITP-NMR) for the separation and analysis of mass-limited samples. In cITP, charged analytes separate based on their electrophoretic mobilities and also concentrate by 2-3 orders of magnitude. The concentrated analytes migrate through the capillary as distinct focused cITP bands and are detected by the NMR microcoil. Applications of this method have been used to study inclusion complexation, chemical analysis of heparin and heparin sulfate oligosaccharides, and impurity levels in the presence of a parent compound. Particularly for the latter, these analytes can then be captured and analyzed using LC-MS-MS/MS for further investigation of additional impurities previously undetected by current pharmaceutical methods. |
|
General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |