Comparison of pH values for two different biochemical buffers from 5 to 55°C

CHED 179

Sean R. LeNoue, slenoue@drury.edu, Cole E. Denton, cdenton@drury.edu, Chandra N. Roy, croy@drury.edu, Colby H. Grove, cgrove@drury.edu, Lakshmi N. Roy, lroy@drury.edu, and Rabindra N. Roy, rroy@drury.edu. Walter H. Hoffman Department of Chemistry, Drury University, 900 N. Benton Ave., Springfield, MO 65802
The control of acidity in the biological pH range 6 to 8 is of importance in clinical chemistry. The buffer compounds HEPPSO and ACES have been studied. Conventional paH values for four physiological buffer solutions have been studied: (i) 0.05 molal ACES + 0.05 molal sodium ACESate; (ii) 0.05 molal ACES + 0.05 molal NaACESate + 0.11 molal NaCl; (iii) 0.08 molal HEPPSO + 0.08 molal NaHEPPSOate; and (iv) 0.08 molal HEPPSO + 0.08 molal NaHEPPSOate + 0.08 molal NaCl; in the temperature range 5 to 55°C and have been assigned based on Pitzer's theory and Bates-Guggenheim convention. The cells were used without liquid junction of the type: Pt; H2(g, 1 atm) | Buffer (m1) + NaBufferate (m2) + NaCl (m3) | AgCl, Ag. The results will be discussed in terms of pH standard data to be used in the study of biomedicine.
 

Undergraduate Research Poster Session: Analytical Chemistry
2:30 PM-4:30 PM, Monday, 11 September 2006 Moscone Center -- Hall D, Poster

Division of Chemical Education

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006