ANYL 38 |
| Spectral simplicity of solution NMR spectra results from the Brownian rotational diffusion of solutes, which rapidly averages the strong dipolar interactions between different spins to exactly zero. Much valuable structural information, contained in these dipolar interactions, is lost in this averaging process. It has long been known that alignment of solutes in a magnetically oriented liquid crystalline medium restores the dipolar interactions, albeit at the cost of dramatically increased spectral complexity, limiting this approach to only very simple systems. However, by decreasing the degree of solute alignment, it is possible to retain the valuable structural information contained in the dipolar couplings, without considerably increasing spectral complexity. The approach is particularly powerful for answering questions in macromolecular chemistry, and examples will be presented for both proteins and nucleic acids.
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NMR Then and Now: Honoring Ted Becker, Recipient of the Analytical Chemistry Service Award Supported by Varian, Inc
2:15 PM-4:45 PM, Sunday, 10 September 2006 Moscone Center -- Room 123, Oral
Division of Analytical Chemistry |