BIOT 15 |
| Selection of resins is a key activity in development of purification processes for new pharmaceutical proteins. Screening of resins for a specific purpose was traditionally performed by selection of a few preferred resins at a number of relevant conditions, and then selection of the most proper resin in light of purity, speed to market and process economy. However, robot techniques are increasingly being implemented as the preferred tool for screening of resins and conditions, as it provides possibilities to test an increasing number of resins and conditions in a fully automated set-up. The scope of this paper is to present the results of a number of commercial weak anion-exchange resins by a systematic and consistent comparison in a traditional experimental set-up. The comparison includes data on efficiency, binding strength, pH dependence, dynamic capacity, and particle size distribution of the ion-exchange resins performed at the same, relevant conditions with model proteins. Relevant data for model proteins will then be compared to data obtained with robot techniques to validate the automated approach. Finally, data on samples from human growth hormone feed-stocks will be presented and related to the data with model proteins.
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Downstream Processing: Advances in Chromatographic Separations
9:00 AM-12:00 PM, Sunday, 10 September 2006 Hilton San Francisco -- Plaza, Oral
Division of Biochemical Technology |