INOR 380 |
| Inhibition of protein tyrosine phosphatase 1B (PTP1B) has been proposed as a novel therapeutic strategy to treat type 2 diabetes and obesity. This study investigates the role of vanadium complexes as inhibitors of protein tyrosine phosphatase 1B. Vanadium compounds with dipeptide Schiff base ligands and other ligands including biguanide, o-phen, biimidazole and benzimidazole have been synthesized. In order to obtain their inhibiting effects, a phosphatase inhibition assay by fluorescence spectroscopy (excitation 360 nm, emission 450 nm) has been used by employing DiFMUP as a substrate. IC50 values were calculated from non-linear regression fit of initial rates of hydrolysis obtained by reacting with 12.6 nM of recombinant PTP1B. The results show that these vanadium compounds can inhibit PTP1B to certain extents. We thank the National Natural Science Foundation of China (grant No. 20471033) and the Natural Science Foundation of Shanxi Province, China (grant No. 20051013) for financial support. |
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5th International Symposium on Chemistry and Biological Chemistry of Vanadium: Posters in Chemistry and Biochemistry
7:00 PM-10:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Inorganic Chemistry |