Study of the interaction in aqueous solution of vanadate with MHCPE, a heterocyclic pyrimidinone derivative ligand, by 51V NMR spectroscopy

INOR 376

Fernando Avecilla, avecil@udc.es1, Lorena Palacio1, A. Figueiredo2, H. Faneca3, João Costa Pessoa, pcjpessoa@mail.ist.utl.pt4, Carlos F. G. C. Geraldes2, MCP. Lima3, and M. Margarida C. A. Castro, gcastro@ci.uc.pt2. (1) Departamento de Química Fundamental, Universidade da Coruña, Campus de Zapateira s/n, A Coruña, 15071, Spain, (2) Departamento de Bioquímica, Centro de Neurociências e Biologia Celular, Centro de RMN, Universidade de Coimbra, Coimbra, 3001-401, Portugal, (3) Departamento de Bioquímica, Centro Neurociências e Biologia Celular, Universidade de Coimbra, Coimbra, Portugal, (4) Centro de Química Estrutural, Instituto Superior Técnico, Av. Rovisco Pais, Lisboa, 1049-001, Portugal
In recent years, vanadium compounds have attracted a lot of interest due to their potential application in the treatment of diabetes. Many V(IV) and V(V) complexes have been synthesized, in a search for desired properties such as hydrolytic stability, water solubility, neutral charge and/or lipophilicity, and their toxicity and insulin mimetic action have been evaluated. In this work we report a study of the interaction of vanadate with a heterocyclic ligand derived from pyrimidinone, MHCPE, 2-methyl-3H-5-hydroxy-6-carboxy-4-pyrimidinone ethyl ester, in aqueous solution, at different M/L ratios and pH values, using 51V NMR spectroscopy. Two main species are formed in solution and the respective formation constants were determined. The toxicity and possible insulin-like activity of these species were also evaluated in the HeLa and 3T3 L1 tumour cell lines.

 

5th International Symposium on Chemistry and Biological Chemistry of Vanadium: Posters in Chemistry and Biochemistry
7:00 PM-10:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster

Division of Inorganic Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006