INOR 144 |
| One approach under investigation in cancer chemotherapy is the irradiation of a high Z atom near the cellular DNA. Upon uptake of a photon of the correct energy, the high Z atom can release a number of low energy Auger or Koster-Kronig electrons, which can damage the DNA. The process is called photon activation therapy (PAT). We report evaluation of a number of transition metals using the tetracationic metalloporphyrin 5,10,15,20-tetrakis(1-methylpyridinium-4-yl) porphyrin (TMPyP4) as the scaffold for the metal. pUC19 plasmid cleavage assays were run to evaluate the potential of these molecules to create clustered DNA damage on irradiation (1 µM concentration of the porphyrin, 20 µM plasmid and 2 mM glycerol). Experiments evaluated the effect of metal, buffer, concentration of glycerol, irradiation time, and concentration on the plasmid cleavage. DNA binding constants for specific metalloporphyrins have been measured by isothermal titration calorimetry (ITC). |
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Bioinorganic Chemistry
7:00 PM-10:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Inorganic Chemistry |