Effect of temperature on gradient reequilibration in reversed-phase liquid chromatography

CHED 183

Brian W. Roe, bwr301@jaguar1.usouthal.edu and Jason W. Coym, jwcoym@jaguar1.usouthal.edu. Department of Chemistry, University of South Alabama, Chemistry 223, Mobile, AL 36688-0002
Column reequilibration after gradient elution has long since been a problem when performing HPLC experiments. Reequilibration after a gradient experiment wastes valuable time and solvents, which has a direct affect on experimental throughput and analysis cost. In this study, reequilibration times of several different stationary phases were examined as a function of temperature. Some of the stationary phases that were examined included a polar embedded C-18 phase, a traditional C-18 phase, and polar end-capped C-18 phase. The temperature range that was studied was from 10°-50°C.