Electrochemical probes for ligand-receptor binding

INOR 196

Amanda L. Eckermann1, Dimitra Georganopoulou2, Barker Kylie1, and Thomas J. Meade, tmeade@northwestern.edu3. (1) Department of Chemistry, Northwestern University, 2145 Sheridan Rd., Evanston, IL 60208, (2) Ohmx Corporation, (3) Department of Chemistry and Department of Biochemistry and Molecular and Cell Biology, Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208
Reorganization energy (λ) in electron transfer comprises properties such as changes in bond distances and angles as well as outer-sphere solvent reorientation. These factors are closely related to ligand-receptor binding in biological systems where the displacement of water and formation of new hydrogen bonds are crucial steps. Electrochemical methods can be used to measure reorganization energy of electrode-bound metal complexes. Therefore, we are employing electrochemistry to study the energetics of ligand receptor binding and have designed a series of avidin-binding ruthenium and iron complexes that can be covalently attached to an electrode. The electrochemical properties of these electrode-attached, transition metal complexes have been measured in the absence and presence of protein and the data have been fit to simulations so that kET and λ can be calculated.
 

Electrochemistry
7:00 PM-10:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster

Division of Inorganic Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006