BIOT 80 |
| Removal of cellular debris present in mammalian cell conditioned media has become increasingly difficult due to high cell density, low viability, and extended cell culture duration. A method of flocculation is described that entails the addition of calcium chloride and potassium phosphate to the cell-containing medium, resulting in a precipitated solid that associates with debris and facilitates its removal by centrifugation. After development at the lab-scale, the method was tested at pilot-scale in several antibody processes. The turbidity in the centrate was significantly reduced with recovery of antibody typically greater than 90%. The flocculation of the cell-containing conditioned media decreased the particulate burden on the subsequent filtration train, decreased the precipitation of the Protein A peak, and, in some cases, increased the removal of host cell proteins. |
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Downstream Processing: Alternatives to Chromatographic Separations
8:00 AM-11:00 AM, Monday, 11 September 2006 Hilton San Francisco -- Plaza, Oral
Division of Biochemical Technology |