ANYL 86 |
| A simple, rapid, selective and sensitive method for the simultaneous determination of amphetamines in real urine sample (using only 20 uL) without pretreatment like extraction or cleanup has been developed. The method uses gas chromatography (GC) direct sample introduction (DSI) device for on-line derivatization (acylation) of amphetamines. Further, laboratory-built multiple chemical ionization (CI) reagent system introduces furan CI reagent which improves selectivity and sensitivity of detection using tandem mass spectrometry. Sharp analyte peaks with relatively low background improved the sensitivity. The limits of detection (LODs) between 0.5 to 1.0 ng mL-1 and limits of quantitation (LOQs) between 1.0 to 2.0 ng mL-1 have been achieved for each amphetamine. Good linearity between 1.0 and 500 ng mL-1 was observed for all analytes with correlation coefficients of r2 > 0.996. Precision and accuracy were also tested to confirm the reliability. Good recovery between 86 and 112% was obtained from five spiked samples. The RSDs range from 5.4 to 18.1%, indicating good repeatability. To the best of our knowledge, presently, this is the most simple, rapid, sensitive, and specific method for the fast screening and determination of amphetamines. |
|
General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |