COLL 29 |
| Previous experiments have suggested that Vaccinium macrocarpon (cranberry) inhibit the adhesion of Escherichia coli to uroepithelium. Experiments were designed to characterize the changes that cranberry juice cocktail (CJC) can impart on bacterial morphology and adhesion. Two strains of E. coli were chosen, HB101 and HB101pDC1. A green fluorescent protein vector was inserted in both strains and bacterial cultures were grown in tryptic soy broth, with 10 wt. % CJC. Replacement of media and continued culture in CJC resulted in morphological changes in both strains. Adherence experiments evaluated if the change in morphology affected the adhesion of bacteria to uroepithelial cells. The number of bacteria adhered to 150 uroepithelial cells was determined by fluorescence microscopy. The number of bacteria that adhered to uroepithelial cells decreased by >50% after exposure to 10 wt% CJC for at least eight culture times. These results scientifically validate the use of cranberry as a preventative measure for UTIs. |
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Structure, Interactions and Reactivity at Microbial Surfaces
8:30 AM-11:40 AM, Sunday, 10 September 2006 Sir Francis Drake -- Windsor Room, Oral
Division of Colloid & Surface Chemistry |