COLL 168 |
| We have explored the possibility to nanoengineer the viral surface by interacting adenovirus (Ad) with lipid bilayers. The interaction of adenovirus with preformed liposomes from dimyristoyl phosphatidylcholine (DMPC) and cholesterol (Chol) was characterized. A high degree of association between liposomes and Ad can be identified by electron microscopy (TEM) analysis. Most strikingly, gene transfer experiments demonstrate that Ad infectivity can be positively and negatively modulated by Ad surface modification achieved by mixing Ad with different concentrations of DMPC:Chol liposomes. Moreover, interaction with model tumor spheroids indicates that deeper penetration and diffusion of the virions can be achieved. These results indicate that it may be possible to engineer viral nanoparticles and alter adenoviral tropism through interaction with zwitterionic phosphtidylcholine and cholesterol containing liposomes. |
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Advances in Nanomedicine
8:30 AM-12:20 PM, Monday, 11 September 2006 Sir Francis Drake -- Monterey/Cypress Rooms, Oral
Division of Colloid & Surface Chemistry |