ANYL 250 |
| Multi-segment silica nanotubes (SNTs) were fabricated using template synthesis for multiplexed bioassays as a dispersible microarray system. Each different shape of nanotube was used as a biomolecular code since the individual nanotube's shape could be easily distinguished by the reflectance and the length of each segment on the nanotube using a conventional optical microscope. The multi-segment silica nanotube may provide a solution for the problems that current microarray systems have, such as spectral overlap or quenching of fluorescence signals and degradation of materials. Multistep anodization technique successfully fabricated well-defined cylindrical pores with two or more (up to five) different diameter segments on the aluminum substrate. SNTs can suspend well in aqueous solution because of their tubular structures, and they have been stable in solution without any visible degradation for six months. Proof-of-concept multiplexed immunoassay results demonstrated that the multi-segment SNTs biosensing system is highly selective; and primary sensitivity experiments showed that the detection limit of the analyte, rabbit IgG, is about 0.3 nM.
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Analytical Approaches: Sensors
8:30 AM-11:40 AM, Wednesday, 13 September 2006 Moscone Center -- Room 123, Oral
Division of Analytical Chemistry |
