High purity purification of lead compounds by supercritical fluid chromatography (SFC) for AMES testing

ANYL 131

Wesley W Barnhart, wesleyb@amgen.com, Kyung H Gahm, Zheng Hua, David Semin, and Janet Cheetham. Discovery Analytical Sciences, Amgen Inc, 1 Amgen Center Dr, 2-M-B, Thousand Oaks, CA 91320
The AMES test is a short-term bacterial reverse mutation genotoxicity assay utilized as an initial screen for determining the mutagenic potential of a new drug candidate. No clear guidelines exist specifying the purity of test compounds for the Ames test at the discovery stage. However, to minimize false positives it is general practice across the industry to ensure the purity of submitted materials is very high (e.g. >99%). Many strategies have been explored to improve efficiency in the method screening and purification of both chiral and achiral compounds. SFC has been found as an efficient and economical tool for purification in drug discovery and facilitates high purity purification of materials. In this poster, several examples are given to show the efficiency and benefits of utilizing SFC to obtain high purity materials for AMES testing.
 

General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006