Electrical properties of artificial bilayer membranes on solid supports

COLL 482

Peter C. Searson, searson@jhu.edu, Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218

Artificial bilayer membranes on solid supports are model systems for biomembranes and provide a platform for devices based on their structural, sensing, and transport functions.  Artificial bilayers incorporating membrane proteins such as pores, ion channels, and receptor proteins, are important in drug discovery, biosensors, and for studying fundamental aspects of cell function.  In fabricating robust platforms for sensing and bioelectronic devices it is essential to determine the electrical properties of the membranes and their components.  Here the electrical properties of hybrid bilayers and supported membranes are discussed.  Hybrid bilayers were formed from self-assembled monolayers of octadecanethiol (ODT) on gold with an outer leaflet of dioleoylphosphatidylcholine (DOPC).  Results are also presented for supported bilayer membranes of dioleoyltrimethylammonium propane formed on silicon.  As an example, Figure 1 shows the potential dependence of the admittance for a Au/ODT/DOPC hybrid bilayer with 9mol% gramicidin in PBS, illustrating diode-like behavior.

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