Imaging of plant and animal tissues by direct laser desorption/ionization mass spectrometry

ANYL 230

Edward S. Yeung, yeung@ameslab.gov1, Sangwon Cha1, Hui Zhang1, Chanan Sluszny1, and Basil Nikolau2. (1) Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, (2) Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, IA 50011
Laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry was applied for the direct analysis of metabolites on intact plant and animal tissues. Cuticular wax compounds were ionized by laser desorption in the presence of colloidal silver, colloidal graphite, or hydroxlated fullerene. Silver-adduct ions were detected on samples from Arabidopsis Thaliana and from maize. Good spot-to-spot reproducibility indicated homogeneous coverage of the sample by the fine colloidal material. The results were consistent with GC-MS analyses of cuticular extracts, thus confirming the feasibility of direct analysis based on this protocol. Molecular masses of the adduct ions correspond well with the known composition of cuticular waxes. Moreover, LDI-TOF gave good estimates of the relative local abundances of a given compound.
 

Honoring Mary Wirth, Recipient of the Spectrochemical Analysis Award
1:30 PM-5:00 PM, Tuesday, 12 September 2006 Moscone Center -- Room 123, Oral

Division of Analytical Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006