ANYL 7 |
| We demonstrate a separation mechanism for large DNA molecules based on their radial migration in capillary electrophoresis with applied hydrodynamic flow (HDF). The direction of radial migration depends on the direction of the applied HDF relative to the electric field. The radial migration velocities are size-dependent, which could be attributed to the different degree of deformation under shear flow. The separation mechanism was proposed and elucidated, and two molecules: λ DNA (48, 502bp) and ΦX-174 RF DNA (5,386 bp) were used to demonstrate the separation. Baseline separation was achieved in 21 minutes. This separation can be performed continuously and thus is applicable to preparative separation. Without the need for gel/polymer or complex instrumentation, this separation technique is much simpler than capillary gel electrophoresis (CGE) and field-flow fractionation (FFF). This technique could also be applicable to the separation of proteins, cells or particles based on the size, shape and deformability. A homogeneous immuoassay scheme can be devised in this manner. Based on the same concept of deformability, we observed that the large DNA migrates faster than the small DNA in free zone electrophoresis, and can thus be separated from each other. |
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Extreme Chromatography and Separations
8:25 AM-12:00 PM, Sunday, 10 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |