Enhancing the longevity of ion selective electrode arrays in bioreactors

BIOT 241

Hajime Fuchida, hajime@wsu.edu1, Daniel C. Rieck, Daniel_Rieck@wsu.edu1, Bernard J. Van Wie, bvanwie@che.wsu.edu1, David A. Kidwell2, and William C. Davis, davisw@wsu.edu3. (1) Department of Chemical Engineering, Washington State University, Pullman, WA 99163, (2) Naval Research Laboratory, Washington, DC, (3) Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99163
On the basis of ion selective electrode calibration curves, the concentrations of three ions, K+, NH4+ and H+, in bioreactor effluents can be determined. An apparent defect, however, is that electrode sensitivity and response tend to decline for such sampling after long periods of usage. A possible explanation for this is inactivation of electrodes due to the adsorption of proteins or cells onto the membrane. The electrodes are exposed to bioreactor effluents containing DMEM, saline, antibody, calf serum and whole and lysed cells and tested for longevity. This presentation will present various methods for assessing electrode inactivation including detection of adsorbed proteins and cells with dyes, and monoclonal antibodies with an ELISA assay. Potential solutions to these problems will also be discussed including the use of blood compatible copolymers.
 

Poster Session
5:30 PM-7:30 PM, Wednesday, 13 September 2006 Moscone Center -- Hall D, Poster

Division of Biochemical Technology

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006