BIOT 79 |
| Downstream processing of monoclonal antibodies typically involves multiple steps; some of them are performed by bead-based column chromatography. In this work membrane adsorbers have been considered as an alternative technology for the capturing step. To this aim membrane matrices have been functionalised with mimetic ligands that exhibit affinity for the Fc portion of antibodies. These new affinity membrane where characterised in terms of binding kinetics, static and dynamic binding capacity, non-specific binding, stability of the stationary phase and ligand leakage. These parameters were determined with pure IgG solutions and the membrane selectivity was tested with complex mixtures like human serum and MAbs from a cell culture supernatant. Results obtained with the same ligand showed that the separation behaviour depends on the membrane matrix and on the activation chemistry used for ligand coupling. The effects of the introduction of a thiophilic spacer arm are under investigation. |
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Downstream Processing: Alternatives to Chromatographic Separations
8:00 AM-11:00 AM, Monday, 11 September 2006 Hilton San Francisco -- Plaza, Oral
Division of Biochemical Technology |