INOR 615 |
| Nitric oxide (NO) is ubiquitous in biological systems, where it mediates both physiological and pathological processes. To visualize NO in cells, we designed a fluorescein-based copper(II) construct that exhibits “turn-on” fluorescence in response to NO in pH 7.0 buffered solutions. Detection of NO is specific over other reactive species including H2O2, HNO, NO2-, NO3-, and ONOO-. Production of NO by constitutive and inducible nitric oxide synthases in neuroblastoma and mouse macrophage cells was monitored in a spatiotemporal manner with the use of this Cu(II)-based probe. These studies demonstrate that detection of NO in a biological context can be achieved with a suitable fluorescent sensor that utilizes nitric oxide chemistry of metal coordination compounds.
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Bioinorganic Chemistry
1:30 PM-5:30 PM, Tuesday, 12 September 2006 Moscone Center -- Room 304, Oral
Division of Inorganic Chemistry |
