BIOT 99 |
| High-throughput screening technology and Design of Experiment approach are extremely useful for the optimization of the large number of components that are typically present in a chemically-defined cell culture medium. We have developped a cell culture platform based on 96-deepwell plates, which uses exclusively off-the-shelf components. Compared to regular 96-well plates in use today, this platform enables representative performance in terms of cell growth and protein expression. Its major component is a flexible liquid handling robot allowing for (1) easy preparation of complex media preparation based on DoE (>15 factors), (2) quick seeding and sampling of cell culture plates and (3) analysis of various parameters related to cell growth, energy metabolism and waste product accumulation. After qualification of the deepwell platform with respect to reference shake flasks and bioreactors, the platform has been integrated in our cell culture medium development projects. This presentation will describe the development of the platform and present data generated during medium development projects, with a focus on the platform capabilities. The advantages and drawbacks of this platform compared to other scaled-down models for cell culture will be discussed. |
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Upstream Processing: Cell Culture Process Development: Advances in Process Engineering
3:00 PM-5:30 PM, Monday, 11 September 2006 Hilton San Francisco -- Imperial A Ballroom, Oral
Division of Biochemical Technology |