ANYL 84 |
| Society has been very concerned with the potential risk to human life and wildlife due to exposure to both natural and synthetic estrogenic chemical residues that may interfere with reproduction and development. Although the concentration of these compounds in environmental samples is low, it has been demonstrated that the nonconjugated estrogens at low concentrations can cause the synthesis and secretion of vitellogenin in male fish. This study describes a reliable and sensitive method for determining five estrogenic compounds by liquid chromatography/tandem mass spectrometry (LC/MS2). Optimization of the ionization process was performed by using electrospray ionization, atmospheric pressure chemical ionization and dopant-assisted atmospheric pressure photoionization methods with different flow rates in the LC/MS2 scan mode. The better ionization sensitivity for the studied compounds was obtained with ESI and dopant-assisted APPI in negative ion mode when using an acetonitrile-water gradient. Both two ionization methods would applied to environmental water samples to compare the ion suppression effect during ionization process. Quantitation of the target compounds will be confirmed in advance with multiple reaction monitoring (MRM) when complicated matrix are presented in real samples. |
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General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Division of Analytical Chemistry |