ANYL 100 |
| The BEADS platform is a PNNL technology platform for sample clean-up and preconcentration for bioassays. Complete bioassays are performed with on-column detection of a fluorescent bioassay signal. Current BEADS systems with on-column detection employ optical fibers for excitation/illumination of the fluorescent reporter and fiber collection of the emitted fluorescence, limiting the number of photons that can be collected. By collecting a larger portion of the fluorescent signal, the detection limits of the on-column bioassay can be reduced. Conversion of the bead-column flow cell to a liquid core waveguide for sensitive on-column detection of fluorescence-based bioassay signals will be covered. The current bead-column flow cell is constructed from FEP Teflon with a refractive index of 1.35. For waveguide conditions to exist, the refractive index of the flow cell contents must be greater than the flow cell. This presentation explores the modification of the refractive index of the core liquid and fabrication of the flow cell from a lower refractive index material. |
|
General Papers
7:00 PM-9:00 PM, Sunday, 10 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Analytical Chemistry |