ANYL 283 |
| A crystalline silica nanoparticle array is used as stationary phase for a fast electrically driven separation of peptides. Unlike electrochromatography, there is no electro-osmotic because the surface of the silica nanoparticles has no charge after C18 modification and endcapping. The hydrophobic interaction between the analytes and the silica surface contributes to the different net electrophoretic mobility between different peptides, which enables fast separation within short separation distances of less than 1 cm. The mobility, plate height and separating mechanism are studied. A peptide mixture, which cannot be resolved by HPLC or CE, is used as test for the silica crystalline array separation. The mixture is separated in 10 seconds over a 2 mm electro-migration distance, which shows significant advantage over the current separation techniques of HPLC and CE. Applications to peptide mixtures for proteomics are currently being investigated. |
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Analytical Approaches: Separation Science
1:30 PM-5:00 PM, Wednesday, 13 September 2006 Moscone Center -- Room 130, Oral
Division of Analytical Chemistry |