ANYL 347 |
| Bisphosphonates with P-C-P structure are broadly studied for the treatment of born diseases. These compounds are extremely hydrophilic with bioanalytical challenges in sample extraction and analytes detection. An approach to achieve highly sensitive and selective quantative analysis of bisphosphonates in human urine and serum by LC-MS/MS is present here. The novelty is a direct on-column derivatization with diazomethane during the solid phase extraction (SPE). The reproducible methylation the bisphosphonate group enabled a greatly simplified purification procedure. The derivatized anylates are rendered high signal to noise ratio in the Electrospray Ionization (ESI) Mass Spectrometer. A low limit of quantitation (LLOQ) at pictogram/mL can be easily achieved using only 100-200mL of sample. |
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Analytical Approaches: Various Techniques and Applications
1:30 PM-4:00 PM, Thursday, 14 September 2006 Moscone Center -- Room 124, Oral
Division of Analytical Chemistry |